Macrophage podosomes assemble at the leading lamella by growth and fragmentation
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منابع مشابه
Macrophage podosomes assemble at the leading lamella by growth and fragmentation
Podosomes are actin- and fimbrin-containing adhesions at the leading edge of macrophages. In cells transfected with beta-actin-ECFP and L-fimbrin-EYFP, quantitative four-dimensional microscopy of podosome assembly shows that new adhesions arise at the cell periphery by one of two mechanisms; de novo podosome assembly, or fission of a precursor podosome into daughter podosomes. The large podosom...
متن کاملPreferential attachment of membrane glycoproteins to the cytoskeleton at the leading edge of lamella
The active forward movement of cells is often associated with the rearward transport of particles over the surfaces of their lamellae. Unlike the rest of the lamella, we found that the leading edge (within 0.5 microns of the cell boundary) is specialized for rearward transport of membrane-bound particles, such as Con A-coated latex microspheres. Using a single-beam optical gradient trap (optica...
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Cells can contact the extracellular matrix (ECM) through a variety of specialized structures. These cell-matrix contacts contain membrane proteins such as integrins, which bind both matrix components and cellular proteins, thus bridging the substratum and the cytoskeleton (reviewed in Adams, 2001). Matrix contacts provide the physical linkage that enables cells to adhere to and migrate on (or t...
متن کاملAnalysis of Actin FLAP Dynamics in the Leading Lamella
BACKGROUND The transport of labeled G-actin from the mid-lamella region to the leading edge in a highly motile malignant rat fibroblast line has been studied using fluorescence localization after photobleaching or FLAP, and the transit times recorded in these experiments were so fast that simple diffusion was deemed an insufficient explanation (see Zicha et al., Science, v. 300, pp. 142-145 [1]...
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Podosomes are actin-based matrix contacts in a variety of cell types, most notably monocytic cells, and are characterized by their ability to lyse extracellular matrix material. Besides their dependence on actin regulation, podosomes are also influenced by microtubules and microtubule-dependent transport processes. Here we describe a novel role for KIF9, a previously little-characterized member...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2003
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.200212037